Two distinct layer-specific dynamics of cortical ensembles during learning of a motor task

Using two-photon imaging of neuronal activity in mouse motor cortex during the acquisition of a self-initiated lever-pull task, Masamizu and colleagues demonstrate that learning is accompanied by a reorganization of ensemble activity in layer 5a. This reorganization correlates with an increase in ensemble prediction of task accuracy. The authors also find that no such changes take place in layer 2/3.