Platelet-poor plasma of athletes is a potent inducer of myogenic differentiation of C2C12 myoblasts
2019
Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor
plasma (PRP) and platelet poor plasma (PPP), have previously been used to
improve muscle regeneration. In this study, six months’ frozen-stored PPP of
individuals who practiced different types of physical exercise was analysed;
it could steer mouse C2C12 myoblast cells towards proliferation, migration
and myogenic differentiation, and it could affect the morphology/shape of
myotubes. Materials and Methods. PPP of male Olympic weightlifters,
football players and professional folk dancers, aged 15-19, was collected 12
h post-training and stored for 6 months at -20°C. C2C12 cell proliferation
was assessed by MTT test, motility by scratch assay, myogenic
differentiation by myotube formation and gelatinase activity by
gel-zymography. Results and Conclusions. PPP induced proliferation and
migration of C2C12 cells. Proliferative capacity was as follows:
weightlifters > dancers > football players; mean migratory capacity was:
weightlifters = dancers > football players. PPP induced formation of
myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football
players and dancers induced formation of elongated myotubes. The mean
myotube area was as follows: football players > dancers > weightlifters. PPP
gelatinolytic activity was observed; it was negatively correlated with C2C12
myoblast proliferation. These results provide general but distinct evidence
that PPP of individuals practicing certain types of exercise can
specifically modify myoblast morphology/function. This is significant for
explaining physiological responses and adaptations to exercise. In
conclusion, long-term, frozen-stored PPP preserves its potential to modify
myoblast morphology and function. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175062]
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