Diplozoons (Monogenea): what is the molecular base of theirinteraction with the host?
2017
The representatives from the class Monogenea are predominantly
ectoparasites of fish. We adopted the experimental organisms -
Eudiplozoon nipponicum (Diplozoidae, Heteronchoinea); a
blood-feeding monogenean inhabiting gills of common carp
(Cyprinus carpio). In contrast to the other groups of
platyhelminths, the identity of genes, the level of their
expression and spectrum of functional protein molecules
produced by these parasites are very limited. The
pyrosequencing methodology (454/Roche, MiSeq/HiSeq) enabled us
to generate robust genomic/trancriptomic data and combined them
with the results of comprehensive mass spectrometry analysis
(Orbitrap-Elite MS System) of excretory-secretory products
isolated from the adult worms. The pipelines of bioinformatic
data processing were optimized and number of functional
molecules was annotated. The reached platform currently
represents significant source of information available for
further experimental research; up to now at least six
functional protein molecules (peptidases and their inhibitors)
have been molecularly / biochemically / immunochemically
characterized.
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