Studies on liver functions under the effect of sequence drugs.

The effects of sequential therapy (mestranol alone and combined with norethisteron acetate and chlormadinon acetate) on liver functions were studied in 2 groups of healthy females (16-32 years of age): the first group consisted of 15 subjects who were given 0.08 mg/day mestranol from the 5th to the 24th day of the cycle and 1 mg/day norethisterone acetate from the 15th to the 24th day of the cycle; the second group of 15 subjects were given 0.08 mg per day mestranol from the 5th to the 24th day of the cycle and 2 mg/day chlormadinon acetate from the 15th to the 24th day of the cycle. Blood samples were collected and Ujoviridine tests (indocyanine green tests) were done 3 times under standarized conditions in both groups during the 1st treatment cycle. Comparison of the 2 forms of sequential treatment using simple variance analysis showed no significant differences between the 2 gestagen phases (norethisteron acetate and chlormadinon acetate) or between the alkaline phosphatase and aminotransferases (the values for these 2 parameters however dropped after norethisterone acetate and rose after chlormadinon acetate). This was attributed to the observation that the 10-day estrogen phase followed by an estrogen-gestagen phase of identical length closely approximate the physiological cycle. The enzymatic results and the ICG half-life indicate that both sequential treatments have no serious toxic effects on the liver functions of healthy fertile women.