Development and validation of derivative spectrophotometric method for simultaneous estimation of Eperisone hydrochloride and Paracetamol in combined tablet dosage form

Simple, accurate, precise and specific spectrophotometric method has been developed for simultaneous determination of Eperisone Hydrochloride (EPE) and Paracetamol (PCM) in its combined tablet dosage form by using methanol as a solvent. The method is second order derivative method in which absorption at 261.88 nm (ZCP of PCM) was used for quantification of EPE and 265.63 nm (ZCP of EPE) for quantification of PCM. Method follows Beer's linearity in the range of 2-10μg/ml for EPE and 10-50μg/ml for PCM. The mean percentage recoveries were found to be in the range of 98.84 - 100.56 % and 99.06 - 100.80 % for EPE and PCM respectively. The proposed method was validated as per ICH guidelines and successfully applied to the estimation of EPE and PCM in combined Tablet dosage form.