Enzyme linked immunosorbent assay kit detecting sodium pentachlorophenate and application of enzyme linked immunosorbent assay kit
2014
The invention provides an enzyme linked immunosorbent assay kit detecting sodium pentachlorophenate. The enzyme linked immunosorbent assay kit detecting sodium pentachlorophenate comprises an elisa plate coated with coating antigens, a sodium pentachlorophenate standard product solution, HRP-conjugated antibodies, sodium pentachlorophenate specific antibodies, a substrate color developing solution, a stop solution, washing liquid and reconstruction fluid. The coating antigens are sodium pentachlorophenate coupling antigens. The HRP-conjugated antibodies are goat-anti-mouse antibodies labeled by enzyme. The invention further discloses a method applying the enzyme linked immunosorbent assay kit to detect sodium pentachlorophenate. The method includes the steps that firstly, samples are preprocessed; then; the kit is used for detection; finally, a detection result is analyzed. The enzyme linked immunosorbent assay kit can be used for detecting the content of sodium pentachlorophenate in shrimp mince, fish flesh, pork and water samples and is easy and convenient to operate, low in cost, high in sensitivity, capable of performing on-site monitoring and suitable for screening a large number of samples.
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