BOVINE T ONGUE ORGAN CULTURE ASSAY FOR T UMORIGENICITY

SUMMARY: Bovine tongue purchased from public retail food distribution centers was used as the substrate onto which tumor cells were inoculated in organ culture. The tongue could be preseved at 4 ° C for several weeks before use in the assay, and it served as an excellent substrate on which tumor cells grew as well as into which they invaded. The system mimics the intramuscular inoculation of tumor cells in vivo, but without the complications of immunologic rejection mechanisms.