[Fingerprints of Curcuma wenyujin by RP-HPLC].
2010
Objective:To establish a mutual mode fingerprint of Curcuma wenyujin for quality control of C.wenyujin.Methods:Waters Symmetry C18 column was used;the mobile phase was methanol-water in a linear gradient elution with the flow rate of 1.0 mL/min,the temperature of column was 30 ℃;the detection wavelength was set at 215 nm.10 batches of C.wenyujin from different places and different time were determined,3 batches of C.phaeocaulis and 3 batches of C.kwangsiensis were determined in the same chromatographic conditions.Results:11 mutual peaks in the fingerprint of the 10 groups of C.wenyujin,and the S peak among them represented germacrone.Conclusion:Curcuma wenyujin′s fingerprints have strong feature and specificity,which can be combined with assaying in the quality control of C.wenyujin.
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