Time periods for the realization of the embryotoxic action of cyclophosphamide and chloridin in vivo and in vitro

: Female rats with 10-day pregnancy were given cyclophosphamide (30 mg/kg) or chloridin (25 mg/kg). Following 15 minutes - 24 hours the embryos were explanted into the cultural medium containing no teratogenic agents indicated. It has been shown that pathological processes in the embryos became irreversible 15 - 30 minutes after the mother was exposed to cyclophosphamide and 6 hours after chloridin exposure. In the other experiments, embryos from intact females were incubated (1 - 4 hours) in the blood serum of rats given the same drug doses and transferred to the serum from intact animals. The times of cyclophosphamide action realization remained unchanged, while those of chloridin decreased to 1 hour.