Comparative studies on anti-thrombus and anti-coagulation effects of Hirudo of different species

2012 
OBJECTIVE To study and compare the anti-thrombus effects and anti-coagulation activities of Hirudinaria manillensis and Whitmania pigra in vivo and in vitro.METHODS The in vitro anti-coagulation activities of the two species of leeches after different processes were evaluated by directly titration on thrombin.The cerebral thrombosis induced by intravenous injection with adrenalin-collagen,caudal vein thrombosis caused by carrageenin and thrombosis formed in arteriovenous shunt models were used to assess the anti-thrombus effects of the two species of leeches.The in vitro anti-coagulation activities of the two species of leeches after different processes were evaluated by cutting-tail method and capillary method.RESULTS The anti-coagulation activity in vitro of Whitmania pigra was quite better than the fresh and dry Hirudinaria manillensis.Fresh and dry Hirudinaria manillensis and Whitmania pigra after different processes had anti-thrombus activities in vivo,and fresh Hirudinaria manillensis had the highest activity while Hirudinaria manillensis after decoction hardly had any activity in vivo and in vitro.CONCLUSION The active constituent of Hirudinaria manillensis is dramaticlly different from Whitmania pigra,moreover,Hirudinaria manillensis has much more bioactivity than Whitmania pigra which is widely used recently.
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    0
    References
    0
    Citations
    NaN
    KQI
    []