Study on Peptide Mimicking Epitope of Adenovirus by Phage Peptide Library

Objective To obtain the 15-mer phage clones displaying the type 3 adenovirus mimic epitope related to neutralizing antibody and observe their protective effects on Hela cells infected with adenovirus. Methods Type 3 adenovirus neutralizing antibody was used to screen phage display 15-mer peptide library. After 3 rounds of biopanning,22 phage clones were selected randomly and added to the Hela cells infected with adenovirus.The pathological changes of the cells were observed by crystal violet staining, and a positive phage clone with signficant protective effect was selected out and sequenced. Results of the 22 phage clones, 8 showed protective effects on the Hela cells infected with type 3 adenovirus, and sequence of one of them was Leu-Val- Gly- Ile-Trp - His-Arg- Leu-Pro- Gly-Ala- His-Arg- Gly- Ala. Conclusion The peptides displayed by phage can mimic the epitope of adenovirus and show protective effects on Hela cells infected with the adenovirus type 3.