Artificial culturing method of lepista sordida mycelium and culturing medium thereof

The invention discloses an artificial culturing method of a lepista sordida mycelium and a culturing medium thereof, wherein the culturing medium comprise the following components according to parts by weight: 30-50 parts of pine needle, 5-12 parts of peptone, 6-12 parts of yeast extract powder, 2.5-10 parts of glucose, and 15-20 parts of agar; the artificial culturing method comprises cleaning and boiling the pine needle in water for 20-30 minutes, filtering to extract the juice, metering 1000ml of pine needle juice, then sequentially adding the peptone, the yeast extract powder, the glucose and the agar, boiling and mixing uniformly, subpackaging the prepared culturing medium into a test tube, covering a rubber plug, sterilizing under the temperature of 120-130 DEG C for 20-30 minutes, then taking out the culturing medium, naturally cooling to 20-30 DEG C, making an inclined plane culturing medium, inoculating a purified lepista sordida strain under the aseptic condition, putting the inoculated culturing medium in a constant temperature incubator, culturing for 5-10 days at the temperature of 20-30 DEG C until the mycelium grows fully in the test tube, and then refrigerating the mycelium. According to the artificial culturing method and the culturing medium, the operation is simple, the culturing period is shortened, no pollutants or wastes are produced, and the yield of the mycelium is high.