Study on bile excretion of scutellarein
2006
Objective: A HPLC-ECD method was established to determine scutellarin in rat bile.Method: The analytical column was Prontosil C_(18)(4.6 mm×250 mm,5 μm),and the mobile phase was consisted of methanol,50 mmol·L~(-1) phosphate buffer(adjusted by phosphoric acid to pH 2.6),and tetrahydrofuran(40∶60∶10),the flow rate was 1.0 mL·min~(-1);the potential electrode voltage was 100 mv.Result: Concentration profile of scutellarin in rat bile was shown in this paper after oral administration of scutellarein.Conclusion: Only scutellarin was detected in rat bile,while both of scutellarin and scutellarein were detected in rat plasma.
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