EFFECTS OF LOW-FREQUENCY ELECTROACUPUNCTURE ON THE IMMUNOLOGIC FUNCTION IN MORPHINE DEPENDENCE RATS

Objective: To observe the effect of low-frequency electroacupuncture (EA) on the immunologic tunc-tion in morphine dependence rats. Methods: Forty SD rats were used in this study. Morphine-dependence model wasestablished by intraperitoneal injection of morphine hydrochloride continuously for 5 days and hastened by administra-tion (i. p) of Naloxone. These rats were randomly divided into control, model, EA and auto-demorphinization groupswith 10 cases being in each group. In EA group, "Guanyuan"(CV 4),"Mingmen"(GV 4), etc. were punctured andstimulated electrically. Positive T lymphocyte subgroups, CD+4 and CD8+ in the peripheral blood were detected with flu-orescence immuno-assay. Results: In model group, sertrn percentage of CD+4 and CD+4/CD+8 decreased considerablyin comparison with those of control group (P＜ 0.01 ); while in EA group, CD+4 level and CD+4/CD+8 increased signifi-cantly compared with those of model group ( P ＜ 0.01); and no significant differences were found between auto-demor-phinization group and model group and between EA and control groups in these two indexes. Conclusion: Low-frequen-cy EA can promote the restoration of the immune function of morphine dependence rats.