Establishment of tissue culture and rapid propagation technology of Helianthus tuberosus.

Helianthus tuberosus(Jerusalem artichoke) tubers with sprout and shoots with a section of these young stem segments were taken as explants,and were cultured on MS basic medium with several plant growth regulating substances in different concentrations.The technology of tissue culture and rapid propagation on Jerusalem artichoke was researched,and finally filtered out the best combination of technical parameters to establish Jerusalem artichoke regeneration system.The results showed that the optimal explants material for rapid propagation were shoots,positive inoculation(morphology lower down) was the optimal inoculation method.The optimal shoot induction medium was MS+1.0 mg·L-1 6-BA +0.2 mg·L-1 IBA with the induction rate of 95%.The optimal subculture medium was MS+2.0 mg·L-1 6-BA,the optimal strong seedling medium was MS+0.1 mg·L-1 6-BA.The optimal rooting medium was MS+0.2 mg·L-1 NAA,the rooting rate reached 100%,transplant survival rate of 95%,field planting survival rate of 95%.