Construction and identification of recombinant retrovirus vector containing human homeobox gene HOXA9
2010
目的 构建携带同源盒基因(homeobox gene)HOXA9的逆转录病毒载体,并建立稳定产毒的包装细胞株.方法 PCR扩增HOXA9基因编码区全长序列克隆至逆转录病毒载体MSCVneo,经酶切、测序鉴定.将重组载体脂质体转染包装细胞系PT67,以G418筛选稳定产毒细胞株.收集病毒悬液并测定病毒滴度.结果 重组逆转录病毒载体MSCVneo经酶切、测序鉴定正确.将筛选所得的高效产毒细胞株命名为...
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