Star Poly(β-amino esters) Obtained from the Combination of Linear Poly(β-amino esters) and Polyethylenimine

Composed of a three-dimensional structure with a central core and multiple radiating linear “arms”, star polymers represent a significant type of branched macromolecular architectures. Due to the spatially defined core–shell-periphery architecture, star polymers have demonstrated their superiority in a variety of biomedical applications such as drug/gene delivery, molecular imaging, antibacterial agents, and so on. In this paper, we report the successful synthesis of a new type of star-shape poly(β-amino esters) with low molecular weight PEI as core and linear PAE (LPAE) as arms. This new star-PAE exhibits low cytotoxicity and high gene transfection efficacy. Star-PAE achieved between 264-fold and 14781-fold higher gene transfection efficiency of primary rat adipose derived mesenchymal stem cells in comparison with studies performed with the individual PEI and LPAE, respectively. The results suggest that star-PAE is a promising nonviral gene delivery vector.