Immobilized nuclease and preparing method thereof

The invention belongs to the field of biological medicines and relates to an immobilized nuclease and a preparing method thereof. The immobilized nuclease comprises a nuclease and a solid-phase vector. The nuclease is connected to groups on the solid-phase vector through covalent bonds. The activity of the immobilized nuclease is higher than 500 U/g. A functional pH range of the immobilized nuclease is 6-8. The proper function temperature of the immobilized nuclease is 37 DEG C. The invention also discloses the preparing method of the immobilized nuclease. The immobilized nuclease is applied in processes of medicine products, avoids nuclease residue in a final product during product separation and purification steps, and improves the product quality.