Langmuir-Blodgett film based membrane for DNA-probe biosensor

Abstract Covalent attachment of DNA to diacetylene film produced on the surface of oxidized silicon by the Langmuir-Blodgett technique has been studied. Coupling of phage M13 DNA with polymer film was carried out by the carbodiimide method and by the formation of linker structures. Sorption capacity and hybridization efficiency determined using the radioactive label 32 P were about 10–20 pg mm −2 and near 100%, respectively.