Extraction and Contents Determination of Polysaccharide in Cordyceps guangdongensis

Jujuba moth,Euzophera batangensis Caradja,is an important insect pest in jujube orchards.It may cause severe damage in jujube orchards and can attack many other plants,such as loquat(Eriobotrya japonica Lindl.),Horsetail Tree(Casuarina equisetifolia L.) and etc.The conventional gas chromatography with electroantennographic detection(GC-EAD) and two-dimensional(GC×GC) gas chromatography using a time-of-flight mass spectrometric detector(TOFMS),were combined to analyze the female sex pheromone gland extract of the borer.The GC-EAD analysis produced two EAD responses in GC areas where no compounds were detected by FID detection.The GC×GC/TOFMS analysis in this area indicated the presence of several chemicals,including(Z9,E12)-tetradeca-9,12-dien-1-ol and(Z9)-tetradec-9-en-1-ol,pheromone components closely related to Euzophera species.The spectral characteristics,retention behavior and the ability to elicit GC-EAD responses implied that both identified unsaturated alcohols were candidates for sex pheromone components of E.batangensis.The GC×GC/TOFMS facilitated the analysis of complex matrices in a high level and would be a great potential tool for analysis of insect pheromones.