Isolation and Identification of Erysipelothrix rhusiopathiae from Arthritis Swine

In order to provide a basis for control of swine erysipelas,swine erysipelas bacillus was isolated and identified.The synovial fluid of arthritis pigs was inoculated to TSA medium.Small transparent colonies were picked and Gram stained,and the G+ small filamentous bacteria were further identified by PCR using 16S rRNA primers of Erysipelothrix rhusiopathiae.Susceptibility of the positive strains was tested and showed that these isolates were sensitive to penicillin,cefotaxime,ceftazidime,ceftriaxone,cefoperazone,cefazolin,amikacin,amoxicillin,clindamycin,tetracycline and ciprofloxacin but completely resistant to neomycin,sulfamethoxazole,gentamicin,amikacin,vancomycin,and furazolidone.Clinical medication guidance should be based on the above results.