Exercise-induced anti-inflammatory effects in overweight/obese women with polycystic ovary syndrome

Abstract Polycystic ovary syndrome (PCOS) is characterized by exacerbated inflammation, which is implicated in cardiometabolic dysfunction. This study aimed to examine the potential effects of acute exercise on inflammatory responses in obese/overweight PCOS women and their controls. Participants underwent a single bout of moderate-intensity aerobic exercise (30 min at ∼65% of VO 2peak ). Blood and muscle samples were collected immediately before (PRE) and 60 min after the exercise session. Cytokines (i.e., IL-1β, IL-6, IL-4, IL-10, TNF-α) were measured both in plasma and in skeletal muscle, and proteins related to inflammatory signaling (IKKα/β and JNK) were assessed in skeletal muscle. At PRE, PCOS showed elevated muscle TNF-α (+62%, p  = 0.0012) and plasma IL-1β (+76%, p  = 0.0010) compared to controls. In PCOS, exercise decreased plasma and muscle TNF-α (−14%, p  = 0.0003 and −46%, p  = 0.0003), as well as increased plasma and muscle IL-4 (+147%, p  = 0.0018 and +62%, p  = 0.0474) and plasma IL-10 (+38%, p  = 0.0029). Additionally, IKKα/β and JNK phosphorylation in skeletal muscle, which was higher in PCOS at PRE, was significantly reduced by exercise (−58%, p p , exercise effect) showed significant, negative associations for plasma IL-1β (r = −0.92, p p  = 0.0100) and IL-6 (r = −0.58, p  = 0.05), and muscle TNF-α (r = −0.95, p p  = 0.005), and JNK (r = −0.94, p