Compatibility of rodent epithelial progenitor cells with biomaterial hydrogels in vitro

Objective To test the interaction of in vitro cultured cells and biological engineering hydrogel materials, providing theoretical basis for the treatment strategy of in vitro cultured cells and biological engineering hydrogel transplantation. Methods Two different components hydrogel Matrigel and PuraMatrix were used as experimental material, and EPCs derived as previous method used as detecting cells. At different concentrations of Matrigel (10%, 20%, 30%) or PuraMatrix (1%, 0.5%, 0.25%), EPCs were cultivated on surface or within the hydrogels. Observe the morphology and detect cell proliferation by Alamar Blue method in different time to determine the cytotoxicity of hydrogel materials. Observe the in vitro angiogenesis ability of EPCs to detect whether cells still have physiological function. Results three concentrations of Matrigel, 30% Matrigel is highest cytotoxicity. Among three PuraMatrix concentration, 1% PuraMatrix is highest cytotoxicity. The cell proliferation rate of EPCs training on surface of Matrigel is high than suspension in Matrigel, and the difference between these two training mode enhanced while the concentration of Matrigel increased. The in vitro angiogenesis ability of EPCs was observed on 20% and 30% Matrigel surface. EPCs descended to the bottom when cultured in or on surface of 10% Matrigel 7 days. The in vitro angiogenesis ability of EPCs was observed in 20% Matrigel, but in 30% Matrigel, EPCs only stretch minority pustute. The cell proliferation rates were no significant differences when EPCs training on surface of or in 0.25% and 0.5% PuraMatrix. But when EPCs training on surface of or in 1% PuraMatrix, the survival rate of cell is very low. EPCs can migrat in 0.25% and 0.5% PuraMatrix when cultured on surface. But in 0.25% PuraMatrix, EPCs attached to the material, but had difficult to perform in vitro angiogenesis. The in vitro angiogenesis ability of EPCs was observed in 0.5% PuraMatrix. Among three densitys of EPCs, density of 107 cell/ml in vitro angiogenesis are more easily. In the hydrogel. Conclusion Matrigel and PuraMatrix in the appropriate concentration (20%) and 0.5% have good compatibility with EPCs and NSCs, and can serve as support, imitate physiological condition, to provide a three-dimensional cell growth environment, non-toxic degradation products, may be more suitable for transplantation treatment. Key words: Endothelial progenitor cells; Hydrogel; Biocompatibility