Application of size-exclusion chromatography to the analysis of shrimp for sulfonamide residues

The determination of several sulfonamide residues from shrimp tissue using size-exclusion chromatography is presented. Shrimp tissue is extracted with ethyl acetate. The extracted solution is evaporated to dryness and the re-dissolved residue is applied to a chromatographic column containing Sephadex LH-20 gel. Cleanup is performed using this size-exclusion procedure. Determination is accomplished utilizing liquid chromatography. Elution of the sulfonamides from a phenyl column is performed with a methanol: acetic acid: counter-ion mobile phase. Recovery of sulfonamide residues from shrimp range from 70 to 100%.