HPLC Method Development and Validation for Estimation of Chlorthalidone in Tablet Dosage Form

The present work relates to development and validation of simple, precise and rapid high-performance liquid chromatographic (HPLC) method for the analysis of Chlorthalidone in tablet dosage form. Method was developed for qualitative and quantitative estimation of Chlorthalidone in tablet dosage form. The chromatographic separation was achieved by using mobile phase 20 mM potassium dihydrogen orthophosphate buffer pH 4.0: methanol (30:70 %v/v) on HiQ Sil C8 (4.6 mm*250 mm* 5μm) column. The mobile phase was pumped at a flow rate of 1.0 ml/min and the eluent was monitored at 230 nm. Retention time was 3.334±0.042 min. Linearity was observed in the concentration range of 5-30 μg/ml with a correlation coefficient (R2) of 0.9915. All the parameters were validated as per ICH guidelines and found to be suitable for routine analysis of drug in pharmaceutical dosage form. Keywords: Chlorthalidone, Quantitative and Qualitative estimation, HPLC.