Isolation and heterologous expression of the Phanerochaete chrysosporium calmodulin gene

Abstract We cloned complementary DNA (cDNA) corresponding to protein_id 10767 using transcripts extracted from a culture expressing ligninolytic enzymes, which is registered as a Ca 2+ -binding protein in the Phanerochaete chrysosporium whole genome database. We used our results to correct an amino acid sequence present in the database. Phylogenetic and southern blot analyses of this re-predicted sequence suggest that this is the sole gene encoding calmodulin ( CaM ) in this fungus. A P. chrysosporium CaM protein (PcCaM) fused to a His-tag was expressed in Escherichia coli . The His-PcCaM showed appropriate CaM features.