The effect of Lasianthus fardii Hance on duck hepatitis B.

[Objective] To study the effect of the ethanolic extract of Lasianthus fardii Hance on duck hepatitis B,and provide a possible experimental basis for developing a new drug for the treatment of hepatitis B.[Methods] Sixty DHBV posi-tive ducklings were screened by Fluorescence Quantitative Polymerase Chain Reaction(FQ-PCR).The infected ducks were di-vided into five groups randomly by lamination stochastic method of distribution.Six groups were administered intragastricly with high-dose ethanolic extract,middle-dose ethanolic extract,low-dose ethanolic extract of Lasianthus fardii Hance,acyclovir(ACV),model-control and normal-control.There were 12 ducklings in every group,and the ducklings had been treated for 14 days.The quantity of DHBV DNA,DHBsAg,ALT and AST in serum were detected before treatment(T0),7days(T7)and 14days(T14)after treatment,and 5 days after stopping treatment(P5).Liver histopathological examination was done on the 5th days after stopping the treatment.[Results] The amount of DHBV DNA and DHBsAg in serum were not obviously differ-ence between treatment group and the control group(P﹥0.05).The activity of ALT and AST was not obviously difference when all of the treatment groups was compared with the model-control group after treatment(P﹥0.05).No obvious difference was ob-served in the liver pathological examination among these six groups.[Conclusion] The three dose of the ethanolic extract of Lasianthus fardii Hance could not inhibit the replication of DHBV and protect the liver function which was damaged by DHBV.