Optimization of Exploded Corn Stover Substrate by Microbial Fermentation for Animal Feed Production

The solid fermentation substrate of exploded corn stover fermented by Aspergillus oryzae for producing animal feed was optimized.The four-factor and three-level orthogonal expe-riment of exploded corn stover(81%-100%),corn meal(0%,2.5% and 5.0%),wheat bran(0%,2.5% and 5.0%),soybean meal(0%,2.5% and 5.0%) and nutrient solution(0%,2.0% and 4.0%) was used in this experiment,and the culture medium was optimized by determining the cellulose degradation,the filter paper cellulose enzyme activity(FPA)and CMCase activity of the fermented product.The result showed that the optimal medium for biodegradation and FPA production of exploded corn stover was 5% corn meal,2.5% wheat bran,5.0% soybean meal and 4.0% nutrient solution.The levels of nutrient solution,corn meal,and soybean meal had significant effects on stover degradation(P0.05).The optimal medium for CMCase production of exploded corn stover was 2.5% corn meal,2.5% wheat bran,5.0% soybean meal and 4.0% nutrient solution.This work obtained the best culture medium for producing biological corn stover feed was 83.5% exploded corn stover,5.0% corn meal,2.5% wheat bran,5.0% soybean meal and 4.0% nutrient solution.