Target nucleic acid amplification by the ligase reaction that fills empty spaces.

It ARISES conformation "FILLING HOLES" ENHANCED CHAIN ​​REACTION ligase (LCR). FILLING HOLES OF CSF is CSF wherein at least one of the probes THIS recessed so as to form a gap between ADJACENT PROBE WHEN hybridize to the target. THE HOLLOW is filled using polymerase and deoxyribonucleotide triphosphates LINKED BEFORE EACH PROBE. Versions Single and double HOLLOW HOLLOW, WHEN ARE DEPENDING ON ONE OR TWO PROBES CUPPED. IMPROVED LIES IN SELECTING AND USING target sequences so that only a single type, or two types, triphosphates OXIRRIBONUCLEOTIDOS FILLING HOLES DOUBLES ARE REQUIRED each being between one and ten bases long, preferably between one and three BASES. They ARE CLAIMED probes having specific sequences for a number of pathogens.