EFFECT OF OBESTATIN ON GLUCOSE HOMEOSTASIS, INSULIN RESISTANCE AND SERUM INSULIN LEVELS IN TYPE 2 DIABETIC RATS

Objective: To determine the effect of obestain administration on plasma glucose levels, serum insulin levels and insulin resistance in type 2 diabetic Sprague Dawely rats.Study Design: Randomized controlled trail.Place and Duration of Study: This study was carried out at Army Medical College Rawalpindi, from April 2013 to July 2013.Material and Methods: This study was a randomized controlled trial conducted at Physiology department, Army Medical College. Forty-five healthy Sprague Dawley rats were randomly divided in to 3 groups i.e. control group (group-I) fed with normal pellet diet (NPD), diabetic group (group-II) and obestatin treated diabetic group (group-III) fed with high fat diet (HFD). Diabetic was induced by single intraperitoneal injection of streptozotocin (35mg/kg). Insulin resistance was determining by HOMA-IR. After Eight weeks, group-II was treated with obestatin (1nm01/100ml intraperitoneally) Blood samples were obtained by terminal intracardic sampling for bioassays of plasma glucose by glucose oxidase method, serum insulin level by ELISA and measurement of HOMA-IR. Mean ± SD was calculated. Statistical significance of differences across the groups was determined by one-way ANOVA by followed by post Hoc Turkey’s test. A p-value <0.05 was considered significant.Results: Obestatin supplementation in diabetic rats showed significant decrease in plasma glucose levels and insulin resistance on comparison with the non-treated control groups. Serum insulin levels were significantly increased in obestatin group when compared to non-treated diabetic group.Conclusion: Obestatin improves the glycemic status in diabetic rats and can be used as an adjunct therapeutic toolin treatment of obesity induced type-II diabetic mellitus.