Combination Therapy of 3-Methylcholanthrene-induced Mammary Carcinoma in Rats: Effect of Chemotherapy, Ovariectomy, and Food Restriction

The growth of mammary carcinoma induced by oral 3-methylcholanthrene in female Wistar rats is retarded by ovariectomy and by food restriction sufficient to produce 12–14% body weight loss. Estradiol benzoate (NSC 9566), 5-fluorouracil (NSC 19893), miracil D (NSC 14574), mitomycin C (NSC 26980), and AB-100 (NSC 37095) showed inhibitory activity. The best tumor inhibition without mortality was elicited by ovariectomy combined with food restriction, and by ovariectomy combined with estradiol benzoate.