Effect of APS on TLR4、NF-κB expression in HUVEC-C injured by patients blood with hypertension disease

Objective To investigate the effects of APS on TLR4、 NF-κB expression and signal pathway in vascular endothelial cells injured by hypertension patients' blood.Methods Human umbilical vein endothelial cells(HUVEC-Cs)were intervened by 10% serum of hypertension patient or healthy human for 24 h.TLR4mRNA expression in HUVEC-Cs was detected by real time PCR.After lipopolysaccharide(LPS)activated HUVEC-Cs were effected by different dosages APS,TLR4 and NF-κB mRNA were detected by real time PCR,TLR4 and NF-κB protein were detected by Western blot.Results After the serum acted on the HUVEC-Cs for 24 h,TLR4mRNA expression was more increased in the hypertension disease group than the healthy group(P0.01).APS showed the ability of reducing TLR4mRNA expression,inhibiting TLR4 protein expression and suppressing IκB protein degeneration in LPS activated HUVEC-Cs in a dose-dependent manner(P0.05,P0.01,P0.001).Conclusion Inflammatory reaction and immunity disorder initiated by TLR-NF-κB signal pathway is one of the influencing mechanisms in the damage of vascular endothelial cells of hypertension disease.APS can reduce the damage.