Cell-mediated cytotoxicity to non-MHC alloantigens on mouse epidermal cells: IV. An alloantigen shared with B cells but not T cells

Abstract Cytotoxic T lymphocytes raised by immunization of C3H mice with AKR epidermal cells (EC) strongly cross-react with AKR lymphocyte (LC) targets, provided the LC are cultured prior to use as target cells. By nylon-wool separation, negative-selection, positive-selection, and cold-target inhibition methods, we have identified B cells as the susceptible LC targets and determined that their antigenicity requires a culture-induced, proliferation-independent change in antigen expression. Questions concerning the nature of the culture-induced event, the identity of the B-cell alloantigen, and its concomitant expression by EC are discussed.