Separation of polynuclear aromatic hydrocarbons and phenols on a new chemically bonded phase for high-performance liquid chromatography

A new bonded liquid-crystal stationary phase for high-performance liquid chromatography is presented. Synthesis entailed reaction of γ-benzyl-L-glutamate-N-carboxyanhydride with aminopropyl LiChrospher 100 NH2. The new stationary phase was characterized by solid-state13C NMR and elemental analysis. Its analytical properties were tested with polynuclear aromatic hydrocarbons (PAH) and phenols. PAH were chromatographed with hexane-chloroform mixtures as mobile phases and were eluted in order of increasing ring number. Total resolution of the 22 PAH studied was achieved by gradient elution. Phenols were chromatographed with a mixture of chloroform and methanol as mobile phase. Small amounts of methanol have a substantial effect on the separation of the phenols. Use of 0.5% (v/v) methanol enabled the separation of 19 phenols and phenol derivatives.