Genotypic Diversity Analysis of Xanthomonas oryzae pv.oryzicola in Southwest China by Rep-PCR

The genetic diversity of 141 strains of Xanthomonas oryzae pv.oryzicola from Yunnan,Guizhou and Sichuan provinces was analyzed polymorphously by Rep-PCR.Primer J3,ERIC and JELIR had better amplified polymorphism,different toxicity strains of the Rep-PCR fingerprints varied widely.Cluster analysis showed that significant genetic diversity exists within the population of Xanthomonas oryzae pv.oryzicola in Southwest China.There was a high degree of correlation between the genetic strains populations of and their pathogenicity.Strains of strong,middle or weak toxicity were roughly classified into different genetic populations.The result suggests that Rep-PCR technique can be used for detecting genetic variation among strains of Xanthomonas oryzae pv.oryzicola and identification of strains.