DNA Intercalation-based Amperometric Biosensor for Chlorpromazine Detection☆

Abstract Chlorpromazine is psychotropic drug, which molecule consisting of three condensed aromatic rings enables intercalation into DNA double helix. In our research we focused on designing biosensor which allows to detect chlorpromazine or other phenotiazines selectively, in samples of complex matrices. The experiments were carried out with DNA-modified gold amperometric sensors, fabricated by direct writing, by means of microdosing robot. The electrochemical detection of chlorpromazine in range 5 μM – 50 μM after 2-minute incubation was performed by linear sweep voltammetry (LSV) at room temperature. Using the developed DNA-modified gold paste electrodes, micromolar concentrations of chlorpromazine in analyzed samples can be detected. In comparison of chlorpromazine detection on bare gold and DNA-modified electrodes, increase of oxidation peak current was observed. Low limit of detection (LOD) of the developed amperometric sensors for chlorpromazine was determined at the level below 0.6 μM. In samples containing electrochemically active interfering substances only oxidation peak for chlorpromazine was present.