Development of a Real-time PCR Method for Detection of Type 2 Porcine Circovirus

One set of primers and a TaqMan probe were designed based on the nucleotide sequence within ORF2 of Porcine circovirus type 2(PCV2).A quantitative TaqMan real-time PCR was developed for detecting PCV2 with highly specificity,reproducibility and more sensitivity than conventional PCR.Standard dilutions allowed absolute quantification of the amount of viral DNA to 1copy/μL,which is 106 times more sensitive than conventional PCR.The result of detecting PCV2 from tissues and sera of pigs with PMWS also confirms the high sensitivity and specificity of this method.This method is probably a powerful enough tool for early diagnosis of PMWS.