Epidermal growth factor (GGF) and tumor promoter 12-0-tetradecanoylphorbol 13-acetate (TPA) stimulate PG synthesis and thymidine incorporation in cultured porcine thyroid cells

Abstract This is the first report to show that epidermal growth factor (EGF) and 12-0-tetradecanoylphorbol 13-acetate (TPA) stimulate the production of PGE 2 and 6-keto PGF 1α , an end metabolite of PGI 2 . in the thyroid gland. In cultured porcine thyroid cells, EGF and TPA stimulate PGE 2 and 6-keto PGF 1α production; the maximum PG levels were obtained after 3–4 h incubation with EGF or TPA; the addition of as little as 10 −11 M EGF or 5×10 −11 M TPA resulted in increases in PGE 2 and 6-keto PGF 1α , and the maximum levels were obtained with 10 −8 –10 −7 M EGF or TPA. This report also shows that EGF and TPA stimulate [ 3 H] thymidine incorporation.