Selection of RAPD Primers for Efficient Fingerprinting in Roses

This study was conducted to select random amplified polymorphic DNA (RAPD) primers for showing reliable polymorphism in roses. The rose genomic DNA concentration for polymerase chain reaction (PCR) was from 1 to 20 ng and annealing temperature was from 35 to 40℃. Taq polymerase concentration was efficient in 1.0 unit. Band patterns were distinguishable in 155 primers out of 191 primers and the 100 primers were reproducible. Twenty primers were selected with distinguishable and over 80% reproducible polymorphism on agarose gel. This study will support Korean rose breeding program and the fingerprints obtained in this study were uploaded on the internet site; http://hanth.jnu.ac.kr.