Evaluation of Microslide Leukocyte Adherence Inhibition Assay

Microslide leukocyte adherence inhibition (LAI) assay has been developed in our laboratory. It has much advantage, especially in reproducibility as compared to other LAI methods such as hemocytometer, tube and microplate techniques. That is, microslide LAI assay was demonstrated to be completely reproducible in triplicated studies and moreover, when mononuclear cell samples from same donors were tested twice within one week interval, similar results were obtained.It was also manifested that no specificity was found among different histological types of lung cancer, in the sense that mononuclear cells from patients with adenocarcinoma responded to the tissue extract of epidermoid or adenoid cystic carcinoma as well as to that of adenocarcinoma of the lung, though lung cancer extract (lung cancer-associated antigen) specifically responded to mononuclear cells from lung cancer patients in the LAI assy.When purified protein derivative (PPD) was utilized as antigen, LAI response correlated to skin reaction in 7 of 8 normal indivisuals, suggesting that T lymphocyte may be involved in LAI phenomenon.