2,4,6-trinitrotoluene as a trigger of oxidative stress in Fagopyrum tataricum callus cells

Effect of 2,4,6-trinitrotoluene (TNT) on callus cells of Tartar buckwheat (Fagopyrum tataricum (L.) Gaertn.) was accompanied by six-electron reduction of ortho- or para-nitro groups of the xenobiotic with the production of 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT). It was discovered that the xenobiotic TNT impairs integrity of cell membrane, which apparently results from its one-electron reduction coupled with production of nitro radical-anion and superoxide anion.