Chemical confirmation of a pentavalent phosphorane in complex with β-phosphoglucomutase

2005 
This communication reports the X-ray crystal structure of the α-d-galactose-1-phosphate complex with that of Lactococcus lactis β-phosphoglucomutase (β-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of β-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the β-glucose 1,6-bisphosphate ligand and rules out the proposal of a β-PGM-glucose-6-phosphate-1-MgF3- complex.
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