Effect of receptor activator for nuclear factor-κB ligand monoclonal antibody on differentiation of regulatory T cells in mice with myeloma

Objective To study the receptor activator for nuclear factor-κB ligand (Rankl) monoclonal antibody on differentiation of regulatory T cells (Treg) in mice with myeloma.Methods Rankl monoclonal antibody was injected through the caudal vein injection intervention people myeloma mice after subcutaneous model,detection tumor local Treg infiltration and Rankl expression,the mice model points blank control group A,high dose group B,low dose group C.Results Antibody intervention,three mice abdominal subcutaneous masses diameter:group A (0.970 ± 0.216) cm,(0.560 ± 0.164) cm group B,C group (0.410 ± 0.152) cm,(P ＜ 0.05) ; Flow cytometry analysis mice peripheral blood Treg ratio:group A (8.19 ± 1.27) ％,group B (6.32 ± 0.74) ％,group C (3.84 ± 0.43) ％,(P ＜ 0.05) ; reverse transcription-polymerase chain reaction (RT-PCR) detection mass local Rankl mRNA expression (Δ Ct value):A group of 0.565 ± 0.029,3.390 ±0.333 group B,C group 1.041 ± 0.083,(P ＜ 0.01).Conclusion Multiple myeloma cells may through the express Rankl promote Treg differentiation,suppress the normal immune,using Rankl monoclonal antibody blocking the signal path can reduce Treg differentiation. Key words: Rankl monoclonal antibody;  Multiple myeloma;  Regulatory T cells