Study of copy number variations of OCT4 and SOX2 pluripotency genes in stomach tumors.

e15095 Background: In spite of widespread reduction in the incidence of stomach cancer, more than 1 million people develop it all over the world. Since mortality from this disease is getting close to morbidity, study of pathogenesis of stomach cancer is an urgent task of oncology. Copy number variation (CNV) is one of the fundamental mechanisms of changes in the expression of oncogenes and tumor suppressor genes in oncotransformation. The purpose of the study was to analyze variations in copy number of the OCT4 and SOX2 genetic loci in tumoral and conditionally healthy stomach tissues. Methods: Stomach tumor tissues of 29 patients with adenocarcinoma, signet ring cell carcinoma and synchronous cancer (adenocarcinoma+signet ring cell carcinoma) were studied. Genomic DNA was extracted with phenol-chloroform method after pathological verification of archival paraffin blocks. Primers were designed using OCT4, SOX2 and B2M reference DNA sequences of NCBI GenBank. RT-qPCR was conducted using thermocycler Bio-Ra...