Isolation of GPV wild strain and detection by PCR

In this assay,two pairs of primers were designed according with DNA sequence from GPV Hl standard strain.Using the two pairs of primers GRPl/GRP2 and GFl/GR2 detecting the GPV wild strain isolated from infected gooses by PCR.The result showed that both of the two pairs of primers GRPl/GRP2 and GFl/GR2 could specifically amplified expected two bounds of 0.6 bp and 1.6 bp.The results of regressive assays showed that latent period was 8 d,lasting 1～3 d after infected,lethal rate was 100 % of GPV wild strain infected.