NMR spectroscopic properties (1H at 500 MHz) of deuterated* ribonucleotide-dimers ApU*, GpC*, partially deuterated 2'-deoxyribonucleotide-dimers d( TpA* ), d(ApT * ), d( GpC * ) and their comparison with natural counterparts (1H-NMR window)

Abstract Pure 1′ # ,2′,3′,4′ # ,5′,5′'- 2 H 6 -ribonucleoside derivatives 10–14 , 1′ # ,2′,″,3′,4′ # ,5′,5″- 2 H 7 -2′-deoxynucleoside blocks 15–18 and their natural-abundance counterparts were used to asemble partially deuterated ribonucleotide-dimers ( ∗ indicates deuteration at 1′ # ,2′,3′,4′ # ,5′,5″( 2 H 6 )): ApU ∗ 21 , GpC ∗ 22 partially deuterated 2′-deoxyribonucleotide-dimers d(TpA ∗ ) 23 , d(ApT ∗ ) 25 , d(GpC ∗ 26 ( ∗ indicates deuteration at 1′ # , 2′,2″,3′,4′ # ,5′,5″( 2 H 7 )) according to the procedure described by Foldesi et al. (Tetradedron, in press). These five partially deuterated oligonucleotides were subsequently compred with their corresponding natural-abundance counterparts by 500 MHz 1 H-NMR spectroscopy to evaluate the actual NMR simplifications achieved in the non-deuterated part ( 1 H-NMR window) as a result of specific deuterium incorporation. Detailed one-dimensional 1 H-NMR (500 MHz), two-dimensional correlation spectra (DQF-COSY and TOCSY) and deuterium isotope effect on the chemical shifts of oligonucleotides have been presented.