HPLC-ELSD content determination method for paeoniflorin and albiflori in red paeony roots

The invention discloses a HPLC-ELSD content determination method for paeoniflorin and albiflori in red paeony roots. The method includes the steps that 1, a test product solution is prepared; 2, comparison product solutions are prepared; 3, detection is performed, namely, the paeoniflorin comparison product solution and the albiflori comparison product solution in the step2 are taken to be detected under the detection conditions of C18 columns, isocratic elution is performed with an acetonitrile-0.05% formic acid aqueous solution as a mobile phase, HPLC-ELSD detection is performed through an ELSD detector, and curves are drawn to obtain standard curve equations; HPLC-ELSD detection is performed on the test product solution in the step1 under the conditions through the ELSD detector, obtained peak areas of the paeoniflorin and the albiflori are substituted into the standard curve linear regression equations, and then the contents of the paeoniflorin and the albiflori in the red paeony roots can be obtained. The method is good in specificity, durability, precision and accuracy and suitable for global quality control over the Chinese herb-red paeony roots.