Биомаркеры формирования нефросклероза при хронической болезни почек у детей

The concept of chronic kidney disease (CKD), which reflects the character and the rate ofprogression of renal disease, determines the need to search for biomarkers of kidney damage in order to optimize the management ofpatients. There are presented results of studies performed in 126 children, aged of 2-18 years, with CKD stages 1-4. Quantitative analysis of the content in the peripheral blood serum endothelin 1-21, Plasminogen activator inhibitor type 1 antigen (AgPAI-I), homocysteine, intercellular adhesion molecules (sVCAM-I, sICAM-I) was performed by enzyme immunoassay (ELISA). For the determination of the total content of nitrites and nitrates, reflecting endogenous production of nitric oxide, colorimetric microplate ELISA method was used. In observed patients there was established a significant increase in serum levels of endothelin-1, homocysteine, antigen PA1-1 and intercellular adhesion molecule possessing vasoconstrictor, protrombogenic and proinflammatory action, with the simultaneous decreasing of the production of nitric oxide over the progression of CKD from the 1st to the 4th stage. These data witness to the involvement of endothelial dysfunction in the formation of CKD and indicate to the possibility of the use of the studied compounds as diagnostic biomarkers of the progression of CKD in children.