Regeneration of plantlet via somatic embryogenesis from hypocotyls of Tartary Buckwheat (Fagopyrum tataricum)

Buckwheat is a well-known minor cereal crops in South Asia. In vitro tissue cultures of buckwheat serve as an important mean for its improvement through genetic transformation as well as induced somaclonal variation. An experiment carried out to develop the efficient protocol for callus induction and plantlet regeneration for hypocotyls of tartary buckwheat. Twenty combinations of plant growth regulators (PGRs) such as 2, 4- Dichlorophenoxyacetic acid/ benzyladenine (2, 4-D/BA), 2, 4- Dichlorophenoxyacetic acid/ benzyladenine/ kinetin ( 2,4-D/BA/KIN), 2, 4- Dichlorophenoxyacetic acid/ 2-isopentenyl adenine/ kinetin (2,4-D/2ip/KIN), 2, 4- Dichlorophenoxyacetic acid/ zeatin (2, 4-D/ZA), 2, 4- Dichlorophenoxyacetic acid/ zeatin riboside/ thidiazuron (2,4-D/ZR, 2,4-D/ZR/TDZ) in MS basal medium were tested on hypocotyls for callus induction under light and dark condition. Callus formation was induced to all combination of PGRs except 2, 4-D 2 mg/L + ZR 1 mg/L + TDZ 2 mg/L under light condition. The best results for callus induction was obtained with 2, 4-D 4 mg/L + BA 1 mg/L under dark and 2,4-D 2.5 mg/L + BA 1.5 mg/L under light condition, which was 86.6 and 90% callus induction rate, respectively. Five combinations of PGRs in MS basal medium were used on somatic embryogenic callus for plantlet regeneration. Somatic embryos resulting regeneration of plantlet were achieved with IAA 1 mg/L + KIN 1 mg/L + BA 2 mg/L + TDZ 0.5 mg/L.