Fructose 2,6-bisphosphate metabolism during megakaryocytic differentiation of K562 and MEG-01 cells

Fructose 2,6-bisphosphate (Fru-2,6-P2) represents the most powerful activator of 6-phosphofructo-l-kinase, rate-limiting enzyme of glycolysis. Fru-2,6-P2 content is tightly regulated and appears to be under the control of different hormones and growth factors, acting either through covalent modification of isoenzymatic forms of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2), the bifunctional enzyme responsible for the synthesis and the degradation of the compound, or through changes in transcription rate and/or in the expression of different isoforms of the enzyme.